A Study of the Absorption and Stability Characteristics of Sodium Amazolene (coomassie Blue) Individually and in Combination with Indocyanine Green

This investigation was carried out to check the absorption and stability characteristics of sodium amazolene (Coomassie Blue) from different lot numbers and to ascertain whether or not it could be combined with indocyanine green (Cardio Green) in vitro without altering the physical characteristics of either dye. These studies demonstrate that a linear relationship exists between optical density and dye concentration for each batch of dye studied, that sodium amazolene is stable in vitro, and that sodium amazolene can be combined with indocyanine green without altering the absorption characteristics of either dye.